Pre IVF Treatment Tests Investigations

Investigations for Male Infertility Treatments

General physical investigation – This is the evaluation test performed by the specialist. The investigation includes questions from the male regarding inherited conditions, illness, chronic health issues, any previous surgery or injury that could affect fertility. The doctor might ask about the sexual habits and frequency.


Semen analysis

The semen specimen can be gathered by different ways. A male can provide his sample by the ejaculation and masturbation keeping the sample into the special container at the centre’s lab. And the second option is a male can give his semen by using a condom (medical special) during intercourse. Once the centre receives the specimen, the lab examines the semen for any issues –sperm count, motility of the sperm and mobility. The sperm must be collected after 2 to 3 days of complete abstinence to determine volume and viscosity of semen and sperm count, motility, swimming speed, and shape.


Male Surgical Testing

Male fertility testing can include surgical procedures such as a testicular biopsy and vasography. These tests are designed to detect blockages, as well as problems with sperm production.

Scrotal Ultrasound

This is a safe and painless assessment of the scrotum. This test is performed when the male is facing testicular pain. Scrotum ultrasound can show the size of the testicles, varicocele (abnormally swollen veins in the scrotum), a cyst in the scrotum, testicular torsion and more.

Testicular Biopsy

During this test, the specialist takes the sample (small piece of the testicle) from the testicle with a needle. If the outcome of the testicular biopsy shows that sperm production is all good and normal, then issue behind the infertility likely is caused by a blockage or another issue with the sperm transport.



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    Investigations for Female Infertility Treatments


    Hysterosalpingography (HSG)

    an x-ray procedure done with contrast dye that looks at the route of sperm from the cervix through the uterus and fallopian tubes.

    Female Surgical Testing

    Infertility may be the result of abnormalities in the reproductive organs. If fertility tests have eliminated such factors as sperm count or hormone levels as the cause of infertility, female diagnostic surgical testing may be indicated.

    Internal examination and ultrasound

    A gynecological checkup would give us additional information regarding the readiness of the uterus for pregnancy and would also help to diagnose pathological conditions.


    A directed physical exam that may include a pelvic ultrasound should be performed. Ultrasound can help us discover abnormalities with the uterus, fallopian tubes and/or ovaries.

    Depending on the individual couple’s situation, various blood tests on either the female or the male may be needed. Blood tests that might be needed include day 3 follicle stimulating hormone (FSH), luteinizing hormone (LH), prolactin, testosterone (T), estradiol (E2), progesterone (P4), 17-hydroxyprogesterone (17-OHP), thyroxin (T4), thyroid stimulating hormone (TSH), lupus anticoagulant (LAC), anti-cardiolipin (ACL), and possibly other tests.

    Follicular fluid is a novel source of gonadal cells for detection of low-grade mosaicism in the ovaries. All women with unexplained infertility undergoing IVF could be studied to determine the chances of a successful pregnancy. If there is evidence of gonadal mosaicism, the chances of a live birth could be low. Mosaicism for the X chromosome detected rapidly by FISH on follicular fluid cells can be one more indication for preimplantation genetic diagnosis (PGD) in the same and subsequent cycles. This test is useful for women with repeated failed IVF, repeated miscarriages, those with chromosomal problems and advanced maternal age.

    Infertility Investigation in India

    The basic infertility investigation is done when you begin your treatment and is usually arranged right after your initial consultation. A basic infertility investigation involves testing of three things:

    • the ‘woman’s hormone status (day 3 blood work),
    • a test of the Fallopian tubes,
    • And a sperm count.

    The whole basic infertility investigation is completed in one cycle.

    The Bottom Line

    Infertility is defined as the incapacity of a pair to conceive after twelve consecutive months of unprotected intercourse. Age is one of the prominent factors that play a crucial role throughout the pregnancy.

    Many elements can have an effect on fertility, such as the presence of sperm and egg at the proper time in a receptive environment. Tests can evaluate those different aspects of fertility.
    Male infertility is diagnosed by the process of semen analysis. The specimen is studied with a microscope to evaluate the sperm count, the morphology of the sperm, and the ability of the sperm to move. If a male is found to be less sperm count, then there is the matter of decreased fertility.

    The woman is examined for egg production by a combination of tests. One looks at the menstruation regularity because this is a great predictor of ovulation. The blood level of progesterone also can be measured at some point of the month, at the side of basal frame temperature.

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    Disclaimer: Under the pre-Conception and Prenatal Diagnostic Techniques (PCPNDT) Act, 1994, prenatal sex determination is banned in India. No test or treatment for sex selection, sex determination, gender selection, gender determination is done in India.

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